The effects of substrate concentration reaction

However, I do not believe the substrate concentrations were significantly different because my repeats were mostly concordant, so a similar amount of oxygen was produced which must mean that there was a similar number of substrate molecules in each concentration. Extension Work This experiment could be improved in a number of ways.

All the active sites are being used so any extra Hydrogen Peroxide molecules will have to wait until an active site becomes available. The theoretical maximum rate of reaction is when all the sites are being used but in reality this theoretical maximum is never reached due to the fact that not all the active sites are being used all the time.

Draw a new graph. However, this too will only have an effect up to a certain concentration, where the Enzyme Concentration is no longer the limiting factor.

I tried to keep this constant by making sure I swirled the conical flask evenly. This is probably due to an experimental error involving one of the factors mentioned above. Lab II focuses on applied principles of population ecology for PG students.

Viruses, though not strictly classed as living organisms, are also studied. Breaking bonds within the enzyme will cause the Active Site to change shape.

Introduction to Enzymes

The line of best fit also suggests values of concentrations which I have not investigated. I solved this by swirling the conical flask constantly throughout the reactions, which seemed to solve the problem, although this meant that the amount of swirling had to be the same in order to ensure a fair test.

When there is a 1: Immunology Virtual Lab II The branch of biomedicine concerned with the structure and function of the immune system, innate and acquired immunity, the bodily distinction of self from no self, and laboratory techniques involving the interaction of antigens with specific antibodies.

For example, the final average volumes of gas were as follows: The focus is on practical skills in using simple electronics to reinforce application of bio-inspired ideas.

This virtual lab is an introductory course for undergraduate students and deals with the storage and retrieval of data from different biological databases like Gene, Pubmed, GEO, TAIR, Prosite etc. There are advantages and disadvantages associated with all three main methods of linearising the data.

As the scale on the pipettes shows the volume to the nearest mm3 the volume of the solutions that I used should be correct to the nearest mm3. This is because at higher concentration there are more molecules of substrate, so more collisions take place, resulting in more enzyme-substrate complexes being formed.

If an enzyme is to be used to determine the concentration of substrate in a sample e. Microbiology Virtual Lab I The study of microorganisms, which are unicellular or cell-cluster microscopic organisms.

If you like this post please consider linking to it using this code: Though in theory, this should be the trend, my results did not demonstrate this pattern. Population ecology Virtual Lab II Population ecology is the study of populations especially population abundance and how they change over time.

The higher the concentration, the more heat will be produced. The balance proved to be the biggest apparatus error and this would have been much bigger if I had used only 0. This change in shape means that the Active Site is less Complementary to the shape of the Substrate, so that it is less likely to catalyse the reaction.

Extracts of this project must not be included in any projects that you submit for marking. Mix well and let sit for about 3 minutes 3.

Factors affecting Enzyme Activity

To prevent this I had to dry out the barrel and syringe before commencing the procedure. It deals with the structures and functions of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules. I tried to keep all the variables except for the concentration of Hydrogen Peroxide the same for all the experiments.

The Lineweaver-Burk double reciprocal plot rearranges the Michaelis-Menten equation as: Substrate Concentration Increasing Substrate Concentration increases the rate of reaction.

The rate of reaction when the enzyme is saturated with substrate is the maximum rate of reaction, Vmax.Effect of Temperature and Substrate Concentration on Catalase Reactions Blake Devivo Biol Lab February 10, Abstract The human body contains thousands of tiny structures called enzymes that perform specific functions.

The graph shows that when the concentration of enzyme is maintained constant, the reaction rate will increase as the amount of substrate is increased. However, at some point, the graph shows that increasing the amount of substrate does not increase the reaction rate.

Introduction to Enzymes

Apr 30,  · The substrate concentration at this point, even if increased, will not affect the rate of reaction because it is the enzyme which is in low concentration. Draw a graph showing what your PREDICTION will be, and write a statement (such as the one below) showing why the graph shows what it fmgm2018.coms: 5.

Reactions with high amounts of substrate are called zero order reactions because rates of reaction are independent of substrate concentration.

The substrate is simply a. In order to study the effect of increasing the enzyme concentration upon the reaction rate, the substrate must be present in an excess amount; i.e., the reaction must be independent of the substrate concentration.

Substrate Concentration It has been shown experimentally that if the amount of the enzyme is kept constant and the substrate concentration is then gradually increased, the reaction velocity will increase until it reaches a maximum.

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The effects of substrate concentration reaction
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